- CD9 · CD63 · CD81 tetraspanins - surface identity confirmed
- Growth factors: EGF, FGF, VEGF, IGF-1, TGF-β
- Anti-inflammatory miRNA: miR-21 · miR-146a
- Neuroprotective cargo: BDNF · GDNF signalling molecules
- Wnt pathway modulators - follicular regeneration
- Immunomodulatory factors: IL-10 · TSG-6 pathway
- Angiogenic mediators - tissue vascularisation
- Extracellular matrix repair proteins
The cryo-sphere format.
Conventional exosome products rely on lyophilisation - a freeze-drying process that subjects vesicles to prolonged thermal and osmotic stress. Research indicates this can compromise lipid bilayer integrity, reduce cargo bioavailability, and create aggregation upon reconstitution. ExoPearls™ represent a fundamentally different approach: purified HMSC exosomes are precision-formed into solid cryo-spheres via controlled liquid nitrogen immersion, preserving native vesicle architecture in a structured crystalline matrix.
Three-step process
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01
Flash-freeze formation
Purified exosomes dropped into liquid nitrogen at a controlled rate, forming uniform 3–4mm spheres that lock biological cargo in a stable amorphous matrix.
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02
Cargo integrity verification
NTA, TEM, ELISA and RNA sequencing confirm particle counts, morphology, surface markers, and miRNA profile per batch.
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03
Ambient stability
Biological activity maintained at ambient temperature for 12 months. Ships via standard courier - no cold chain, dry ice, or special packaging required.
“Systemic administration of MSC-derived exosomes promotes neuroplasticity and neurological functional recovery. The microRNA cargo within exosomes is largely responsible for these therapeutic effects.”- Frontiers in Cellular Neuroscience, 2024
How ExoPearls™ work.
From source cell to target tissue - a three-stage delivery pathway.
Cell Secretion
HMSCs from Wharton’s Jelly secrete exosome vesicles (30–150nm) carrying growth factors, immunomodulatory miRNA, and neurotrophic proteins.
Cryo-Sphere Preservation
Flash-formed into solid ExoPearl™ spheres via liquid nitrogen - preserving native vesicle architecture with zero cryoprotectant additives.
Target Cell Delivery
Intact exosomes fuse with target cell membranes, delivering miRNA and growth factor cargo directly into recipient cells.
ExoPearls™ vs. Lyophilised Formats
Research indicates that lyophilisation subjects exosomes to prolonged thermal and osmotic stress that can compromise lipid bilayer integrity and reduce cargo bioavailability. The freeze-drying process may cause membrane deformation, vesicle aggregation, and loss of surface marker functionality.
The ExoPearl™ cryo-formation process takes a fundamentally different approach - maintaining intact vesicle structure and biological cargo through controlled liquid nitrogen immersion.
- No reconstitution time - instant dissolution on contact with saline
- No aggregation risk - vesicles maintain individual morphology
- Zero cryoprotectant additives in the final product
- Ambient stability verified across full 12-month shelf life
| Parameter | Lyophilised | ExoPearls™ |
|---|---|---|
| Formation process | Freeze-drying (thermal stress) | Cryo-sphere (LN₂ immersion) |
| Vesicle integrity | Potential bilayer compromise | Native architecture preserved |
| Reconstitution | Requires mixing, variable time | Instant dissolution |
| Cryoprotectants | Often required (trehalose, etc.) | Zero additives |
| Shelf life | Variable, often cold-chain | 12 months ambient |
The right exosome is everything. The wrong one is nothing.
Exosomes do not work as a category. Specific exosomes work - because of their source cell, their cargo profile, their membrane integrity, and the conditions under which they were produced. An exosome from a high-passage, poorly characterised cell line carries a fundamentally different biological message from one secreted by a verified early-passage HMSC from Wharton’s Jelly.
Research indicates that cargo composition, vesicle morphology, and lipid bilayer integrity are the variables that determine biological activity. Production conditions, characterisation rigour, and preservation methodology are not peripheral details - they are the product itself. Without full characterisation, an exosome product is an unverified variable.
HMSC-derived exosomes from verified Wharton’s Jelly donors carry a specific, well-characterised cargo profile - growth factors, immunomodulatory miRNA, neurotrophic proteins, and angiogenic mediators that reflect the regenerative properties of the source cell.
An intact lipid bilayer is required for membrane fusion and cargo delivery. Research indicates that compromised vesicle architecture reduces the ability of exosomes to fuse with target cell membranes and deliver their biological payload effectively.
Full characterisation - NTA, TEM, ELISA tetraspanin panel, RNA sequencing, sterility, endotoxin - is the minimum standard. Batch-level traceability and published COA data are not differentiators; they are baseline requirements for any investigational biologic.
ExoPearls™ represent a significant advance in exosome preservation.
Every sphere is characterised. Every batch is verified. The investigational ExoPearl™ platform is built on the principle that preservation methodology is inseparable from biological outcome.
Licensed technology.
ExoPearls™ are supplied under exclusive licence from the patent holder. The underlying intellectual property covers the cryo-sphere formation process, cargo preservation methodology, and ambient stability technology.
Product Formulations
Three ExoPearl™ variants - BEXO·CORE, BEXO·PRIME, and BEXO·NEURO - each designed for specific investigational applications.
View product formulationsGMP Manufacturing
GMP-certified production from verified donor HMSCs - environmental controls, process validation, and full batch documentation.
Read about GMP manufacturingCharacterisation Panel
NTA, TEM, ELISA tetraspanin panel, RNA sequencing, sterility, and endotoxin testing - the full batch characterisation methodology.
See the characterisation panel